Licor support with contact info and links to technical. Odyssey infrared imaging system operators manual version 3. This protocol is not applicable to proteome profiler arrays that use an hrpconjugated detection antibody instead of streptavidinhrp. Licor biosciences, odyssey infrared imaging system b446. The control sample run on every gel was used to normalize each band on the blot and full blots are available in the supplementary datasheet s1. The odyssey clx system requires an area approximately 54 cm w 21. Being able to double label with the 700 and 800 ir laser also allows us to save blots and time. Originally developed for chemiluminescent detection, many proteome profiler arrays are amenable to nearinfrared nir fluorescence detection using the licor odyssey infrared imaging system. C4 photosynthesis application page licor environmental. Abnormal activity of the mapk and campassociated signaling. Plus links to technical resources, such as manuals, application notes, and the product catalog. It is an absolute, nondispersive infrared ndir gas analyzer based upon a single path. However, the initial reaction in c 4 plants occurs in the mesophyll cells.
Odyssey imagers, pearl imagers, cdigit blot scanner, and aerius imager. Both the top and bottom chambers have propafilm windows and leaf temperature is computed using energy balance. Methylseleninic acid enhances paclitaxel efficacy for the. In the prompt box that opens, select browse from the right side. If you loaded the odyssey prestained molecular weight marker licor, pn 92840000, it w ill be visible in the 700 nm. This finding is in comparison with performing both incubations in pbs tween alone.
Please visit the main page of odyssey software on software. Blots were washed in pbs and then water before image acquisition on the licor odyssey. High doses of ma activate caspase 3, upregulate p53, and produce dna fragmentation and cell death in dopaminergic neurons cadet et al. Licor odyssey sa infrared imaging system biosurplus. This protocol may work on other imaging systems that can detect in the near ir spectrum. Understanding the co 2concentrating mechanism of c 4 plants is becoming increasingly important as scientists strive to meet global needs for fuel and food. I want to analyze them, doing a density intensity plot over a sample lane. We continue to provide chambers, light sources, service, and support for li6400xts. Odyssey with the capability for both infrared fluorescence and chemi would probably be your best bet. Feb 14, 2012 immunereactive bands were quantitated by densitometry using the licor odyssey software and normalized to glyceraldehyde 3 phosphate dehydrogenase gapdh. Plates were then imaged at 700 nm and 800 nm wavelengths using a licor odyssey nearinfrared scanner and licor odyssey software. For more info, see the software specs you can use image studio lite software for basic western blot analysis, but it doesnt operate licor imagers or unicorns. The instrument should be placed on a laboratory bench that is sufficiently sturdy to bear its weight. This video tutorial demonstrates how the image studio 4.
Image studio is compatible with both mac and pc computers. Odyssey blocking buffer tbs from licor biosciences. Licor odyssey western blots hi everyone, i have western blot pictures, either as a two channel picture or two separate pictures of the two channels 700800nm. Odyssey fc dualmode imaging system from licor biosciences description the odyssey fc system allows your lab to combine the proven advantages of quantitative infrared fluorescence detection with simple, streamlined chemiluminescence detection on one instrument. Odyssey infrared imaging system very accurate quantification for western blots due to a wide.
David j reiner nih public access 2 seongjin yu1,2 hui. Densitometry was used to analyze the bands licor odyssey software version 3. Licor, odyssey, mpx, incell western, and irdye trademarks contained in the software product are. Used licor odyssey sa infrared imaging system 3014309.
The chamber is useful for measuring small leaves that are tightly bunched in rosettes and. Molecular imaging on the licor odyssey imaging system mousepod. We also use it for emsa, cell migration, coomassie stained gel pictures etc. I recently found that using the licor odyssey blocking buffer pbs with 0. Automated western blot infrared imaging system model 9260. Our school recently purchased a western blot documentation system to start slowly moving away from film. After providing your information, youll get an email with a link to download image studio lite software. Two different wavelengths for western blot fluorescent dyes 680 and 800nm and pcr gels dyed with gel red detection 600 nm are available. Laser safety the center for devices and radiological health cdrh was established in october, 1982, by the u. The detection quality is excellent and the software is both easy to use and provides clear quantification. The odyssey imager should not be used with any radioactive materials. The odyssey infrared imaging system weighs approximately 33 kg 72 lb.
Moving the odyssey clx imager always be sure to disconnect the power to the instrument before moving it as described in disconnecting the power in chapter 2. Odyssey clx infrared imaging system operators manual. Grow cells in 96well or 384well plates until confluent by standard procedures. The li820 co2 analyzer is a low cost, low maintenance co2 analyzer designed for continuous monitoring applications. Li6400xt portable photosynthesis system licor environmental. The major advantage with this solution is accurate quantification over a broad dynamic range, up to 6 orders with high sensitivity. For each well, the integrated signal intensity in the 700 nm nuclear and wholecell stain and 800 nm phosphos473 akt. Odysseyclximager safetyinterlocks interlocks figure14. Licor odyssey clx the new odyssey clx infra red imaging system from licor biosciences improves your quantitative analysis for detecting proteins on membranes, in cells, in tissue, invivo etc. Please visit the main page of odyssey software on software informer. For other countries, contact licor biosciences or a local distributor. Licor image studio software for accurate data quantification.
Download image studio lite software licor biosciences. Odyssey clx infrared imaging system from licor biosciences. The 640015 extended reach 1 cm chamber is designed with a 1. Dna dot blotting and licor odyssey infrared detection.
Safety considerations chemical safety licor biosciences recommends that all chemicals be handled carefully, and that safe laboratory procedures be followed at all. Be aware of the hazards associated with any chemical before using it. Licor ir based system is obviously elegant system and more quantitative. Mar 04, 2014 this video tutorial demonstrates the acquire ribbon, which contains all of the controls for acquiring new images with image studio 4. Order support, sales support, customer support from licor. Image studio software can be used for quick acquisition and organization of images from most licor imaging systems, including the odyssey clx. Frontiers experiencedependent changes in myelin basic. The licor odyssey fc imaging system is easy to use and the operation is selfexplanatory. With the hood fully open, the odyssey clx imager requires 74 cm 29 of vertical clearance. Twofold serial dilutions of labeled antibody 6 ng to 0.
Odyssey fc dualmode imaging system from licor biosciences. It has two lasers, which operate at 680 and 780nm, for the detection of infrared fluorescent dyes. Multiuser licenses are available so that you can use image studio on multiple computers. Although the lite version is free, there is a more comprehensive paid version of the software that aims to easily integrate with the apparatus that licor also sells. This video tutorial demonstrates the acquire ribbon, which contains all of the controls for acquiring new images with image studio 4. Early versions of the odyssey imager were limited to a 2. To achieve this adaptation, the hrpconjugated streptavidin provided in the kit is simply replaced with irdye 800cw streptavidin, and the arrays are.
Odyssey licor manuals manuals and user guides for odyssey licor. Connecting to a network using an ethernet switch 59. These include such functions as quantification, molecular weight. What is the best detection system for western blots odyssey clx but cant fit it in the budget. As in c 3 photosynthesis, c 4 plants use ribulose 1,5bisphophate carboxylaseoxygenase rubisco as the carboxylation enzyme for the formation of metabolic carbon. Since the odyssey is a two channel instrument it can record data for either wavelength separately or both wavelengths simultaneously. More details about the software image studio lite 5.
Mar 04, 2014 this video tutorial demonstrates the image studio 4. Image studio lite software is compatible with mac and windows. The membranes were blocked with licor blocking buffer lincoln, ne for 1 h at room temperature, then probed with primary antisera supplementary table 3 diluted in 0. The simple interface puts highquality data at your fingertips with a few simple clicks and without manual camera adjustments or multiple exposures. Troubleshooting for the odyssey infrared imaging system.
Image studio lite is a free software package from licor biosciences aimed at life scientists that want to analyze gels, western blots, dot blots, and other similar lab outputs. The same intuitive acquisition software is compatible with these licor imaging systems. Read this entire manual before using the odyssey fc imager. These are products by a single manufacturer or a list of manufacturers. When initial screen has loaded, click on file and select new from the dropdown menu. There are certainly many different ways to both organize and retrieve. Does anyone have experiences with both the licor odyssey.
Odyssey blots are cheaper vs chemi blots but it would be nice to have to the option to do. This simplification not only saves you productivity time, it also conserves water, and reduces the hazardous waste. Licor biosciences recommends that all biochemicals be handled carefully, and that safe laboratory procedures be followed at all times. We demod the licor odyssey, biorad chemidoc, and the syngene pxi. Total protein analysis as a reliable loading control for. Which is the best and more sensitive western blot imaging. This video tutorial demonstrates the image studio 4. To quantify this blot, the blot must be rescanned using a lower. Use of proteome profiler arrays with licor detection. Topbottom before analysis with the odyssey software version 3. Page 4 western blot analysis molecular weight marker if you loaded the odyssey twocolor molecular weight marker licor, pn 92840001 on your gel before transfer, it will be visible in both 700 and 800 nm channels. This protocol is known to work for the licor odyssey classic, odyssey clx, and odyssey sa imaging systems.
The licor odyssey allows us to veiw our western blots quickly and efficiently. However, the initial reaction in c 4 plants occurs in the mesophyll cells, where co 2 is fixed to pyruvate by phosphoenolpyruvate carboxylase pepc. Locationofinterlockson theodysseyclxinstrumentbezel. Licor lab instruments, equipment, and accessories biosurplus. The odyssey fc imager is intended to be used by qualified personnel. B the amount of utrophin and syntrophins examined was assessed by fluorescence intensity quantification with licor odyssey software n 7. The major advantage with this solution is accurate quantification over a. For conformity information, contact licor support at biosupport. The antibodies for the active forms of caspase 3 catalog number 9664 and caspase7 catalog number 9491, and cleaved parp catalog number 5625 were obtained from cell signaling. Introduction space requirements the cdigit blot scanner requires an area 27.
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